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pcmv ha n vector  (TaKaRa)


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    Structured Review

    TaKaRa pcmv ha n vector
    Pcmv Ha N Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 764 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv+n+ha+vector/pCMV-HA+Vector+Set/10__3389_slash_av__2026__15087-99-9-11
    Average 96 stars, based on 764 article reviews
    pcmv ha n vector - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: SDCBP/Syntenin-1 stabilizes BACH1 by disassembling the SCF FBXO22 –BACH1 complex in triple-negative breast cancer
    Article Snippet: pCMV-N-HA vector , Takara Bio USA, Inc. , Cat635690.

    Plasmid Preparation:

    Article Title: Orf Virus ORF120 Protein Positively Regulates the NF-κB Pathway by Interacting with G3BP1
    Article Snippet: .. The HA-ORF120 plasmid was generated by subcloning the ORF120 coding sequence into a pCMV-N-HA vector (Clontech). .. G3BP1 was amplified by PCR and inserted into a pcDNA3.1-mCherry vector (Invitrogen) and a pCMV-N-myc vector (Clontech).

    Article Title: Bioorthogonal chemical reporters for profiling retinoic acid-modified and retinoic acid-interacting proteins.
    Article Snippet: Vitamin A and its primary active derivative, all-trans retinoic acid (RA), are endogenous signaling molecules essential for numerous biological processes, including cell proliferation, differentiation, and immune modulation.. Owing to its differentiation-inducing effect, RA was the first differentiating agent approved for the clinical treatment of acute myeloid leukemia.. While the classical mechanisms of RA signaling involve nuclear receptors, such as retinoic acid receptors (RARs), emerging evidence suggests that RA also engages in non-covalent and covalent interactions with a broader range of proteins.

    Article Title: Transcriptional changes in LMH cells induced by Eimeria tenella rhoptry kinase family protein 17
    Article Snippet: .. The pCMV-N-HA vector and the previously constructed pET30a-EtROP17 were double digested with BamHI and EcoRI , and the nucleotide sequence encoding the matured peptide of EtROP17 ( ) was ligated into the linearized vector pCMV-N-HA using the T4 DNA Ligase (Takara, Dalian, China). .. Following transformation into Escherichia coli DH5α competent cells (Transgen Biotech, China), single bacterial colony was randomly selected for PCR analysis.

    Article Title: Single-cell transcriptomics of pediatric Burkitt lymphoma reveals intra-tumor heterogeneity and markers of therapy resistance
    Article Snippet: For transient expression in HEK-293T cells, polyethylenimine (PEI) (Polysciences) was 106 used as previously described12. .. 107 The pCMV-HA-TPM2.1 plasmid was generated by subcloning the PCR-amplified TPM2.1 108 cDNA (NM_213674) into the pCMV-N-HA vector (Clontech) using SalI and KpnI restriction sites. ..

    Article Title: Genistein is effective in inhibiting Orf virus infection in vitro by targeting viral RNA polymerase subunit RPO30 protein.
    Article Snippet: .. Gene amplication, plasmid construction, and antibody preparation The ORFV RPO30 gene was amplified from viral genomic DNA extracted from OFTu cells infected with ORFV strain OV-SY17 and then inserted into pCMV-N-HA vector (Clontech, United States) at the EcoRI and BglII sites to generate pCMV-N-HA-RPO30 plasmid. ..

    Generated:

    Article Title: Orf Virus ORF120 Protein Positively Regulates the NF-κB Pathway by Interacting with G3BP1
    Article Snippet: .. The HA-ORF120 plasmid was generated by subcloning the ORF120 coding sequence into a pCMV-N-HA vector (Clontech). .. G3BP1 was amplified by PCR and inserted into a pcDNA3.1-mCherry vector (Invitrogen) and a pCMV-N-myc vector (Clontech).

    Article Title: Single-cell transcriptomics of pediatric Burkitt lymphoma reveals intra-tumor heterogeneity and markers of therapy resistance
    Article Snippet: For transient expression in HEK-293T cells, polyethylenimine (PEI) (Polysciences) was 106 used as previously described12. .. 107 The pCMV-HA-TPM2.1 plasmid was generated by subcloning the PCR-amplified TPM2.1 108 cDNA (NM_213674) into the pCMV-N-HA vector (Clontech) using SalI and KpnI restriction sites. ..

    Subcloning:

    Article Title: Orf Virus ORF120 Protein Positively Regulates the NF-κB Pathway by Interacting with G3BP1
    Article Snippet: .. The HA-ORF120 plasmid was generated by subcloning the ORF120 coding sequence into a pCMV-N-HA vector (Clontech). .. G3BP1 was amplified by PCR and inserted into a pcDNA3.1-mCherry vector (Invitrogen) and a pCMV-N-myc vector (Clontech).

    Article Title: Single-cell transcriptomics of pediatric Burkitt lymphoma reveals intra-tumor heterogeneity and markers of therapy resistance
    Article Snippet: For transient expression in HEK-293T cells, polyethylenimine (PEI) (Polysciences) was 106 used as previously described12. .. 107 The pCMV-HA-TPM2.1 plasmid was generated by subcloning the PCR-amplified TPM2.1 108 cDNA (NM_213674) into the pCMV-N-HA vector (Clontech) using SalI and KpnI restriction sites. ..

    Sequencing:

    Article Title: Orf Virus ORF120 Protein Positively Regulates the NF-κB Pathway by Interacting with G3BP1
    Article Snippet: .. The HA-ORF120 plasmid was generated by subcloning the ORF120 coding sequence into a pCMV-N-HA vector (Clontech). .. G3BP1 was amplified by PCR and inserted into a pcDNA3.1-mCherry vector (Invitrogen) and a pCMV-N-myc vector (Clontech).

    Article Title: Transcriptional changes in LMH cells induced by Eimeria tenella rhoptry kinase family protein 17
    Article Snippet: .. The pCMV-N-HA vector and the previously constructed pET30a-EtROP17 were double digested with BamHI and EcoRI , and the nucleotide sequence encoding the matured peptide of EtROP17 ( ) was ligated into the linearized vector pCMV-N-HA using the T4 DNA Ligase (Takara, Dalian, China). .. Following transformation into Escherichia coli DH5α competent cells (Transgen Biotech, China), single bacterial colony was randomly selected for PCR analysis.

    Clone Assay:

    Article Title: Bioorthogonal chemical reporters for profiling retinoic acid-modified and retinoic acid-interacting proteins.
    Article Snippet: Vitamin A and its primary active derivative, all-trans retinoic acid (RA), are endogenous signaling molecules essential for numerous biological processes, including cell proliferation, differentiation, and immune modulation.. Owing to its differentiation-inducing effect, RA was the first differentiating agent approved for the clinical treatment of acute myeloid leukemia.. While the classical mechanisms of RA signaling involve nuclear receptors, such as retinoic acid receptors (RARs), emerging evidence suggests that RA also engages in non-covalent and covalent interactions with a broader range of proteins.

    Construct:

    Article Title: Transcriptional changes in LMH cells induced by Eimeria tenella rhoptry kinase family protein 17
    Article Snippet: .. The pCMV-N-HA vector and the previously constructed pET30a-EtROP17 were double digested with BamHI and EcoRI , and the nucleotide sequence encoding the matured peptide of EtROP17 ( ) was ligated into the linearized vector pCMV-N-HA using the T4 DNA Ligase (Takara, Dalian, China). .. Following transformation into Escherichia coli DH5α competent cells (Transgen Biotech, China), single bacterial colony was randomly selected for PCR analysis.

    Polymerase Chain Reaction:

    Article Title: Single-cell transcriptomics of pediatric Burkitt lymphoma reveals intra-tumor heterogeneity and markers of therapy resistance
    Article Snippet: For transient expression in HEK-293T cells, polyethylenimine (PEI) (Polysciences) was 106 used as previously described12. .. 107 The pCMV-HA-TPM2.1 plasmid was generated by subcloning the PCR-amplified TPM2.1 108 cDNA (NM_213674) into the pCMV-N-HA vector (Clontech) using SalI and KpnI restriction sites. ..

    Amplification:

    Article Title: Genistein is effective in inhibiting Orf virus infection in vitro by targeting viral RNA polymerase subunit RPO30 protein.
    Article Snippet: .. Gene amplication, plasmid construction, and antibody preparation The ORFV RPO30 gene was amplified from viral genomic DNA extracted from OFTu cells infected with ORFV strain OV-SY17 and then inserted into pCMV-N-HA vector (Clontech, United States) at the EcoRI and BglII sites to generate pCMV-N-HA-RPO30 plasmid. ..

    Infection:

    Article Title: Genistein is effective in inhibiting Orf virus infection in vitro by targeting viral RNA polymerase subunit RPO30 protein.
    Article Snippet: .. Gene amplication, plasmid construction, and antibody preparation The ORFV RPO30 gene was amplified from viral genomic DNA extracted from OFTu cells infected with ORFV strain OV-SY17 and then inserted into pCMV-N-HA vector (Clontech, United States) at the EcoRI and BglII sites to generate pCMV-N-HA-RPO30 plasmid. ..



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